Journal: medRxiv
Article Title: CLIA MDK: A Modular Smartphone Platform Matching Plate Reader Performance for Chemiluminescent Immunoassay Development
doi: 10.64898/2026.03.26.26348440
Figure Lengend Snippet: The effect of image acquisition and processing on the lower limit of detection (LLoD), limit of quantification (LoQ), and upper limit of detection (ULoD) on two CLIAs quantified with MR and X13P. a Average spectral response of smartphone camera sensors in blue (line), green (line), and red (line) channels (data taken form ) with Luminol emission spectrum (black line) and its peak and center wavelength (vertical dotted dashed line and dashed line). b Primary color channel-based dynamic range for a renin assay at different concentrations with the average signal (black dot, vertical line (mean ± SD), n = 2) normalized to the LLoD (vertical orange line) with LoQ (vertical violet line), ULoD (vertical pink line) and sensor saturation (vertical dashed red line, at 16-bit). The grey box indicates the range between LLoD and ULoD. The further apart the data and limits, the larger the dynamic range. c Same as in b with the green channel and different ISO values (1600, 3200, 12800). d Same as in b with the green channel, ISO 1600 for different exposure times (t exp = 5, 15, 30 s). e Same as in b with the green channel, ISO = 1600, t exp = 5 s for unprocessed (RAW), ROF denoising (ROF), NREA on RAW or ROF denoised images (NREA RAW , NREA ROF ) for one and a series of six images. f Standard curve for renin for RAW (dark blue dots, vertical bar, mean ± SD), and NREA ROF (light blue dots, vertical bar, mean ± SD) with LLoD, LoQ, and ULoD (vertical orange, violet, and pink line, dotted RAW, solid NREA ROF ). The inset shows the zoomed-in region between 0 and 50 pg mL −1 , visualizing the improvement in sensitivity of NREA ROF between 0 and 10 pg mL −1 compared to the other condition. g Same as in f for the standard curve of the aldosterone assay.
Article Snippet: We evaluated smartphone cameras (Xiaomi 13 Pro, Huawei P60 Pro, P20, Fairphone 5, Nokia G22) and CSI cameras (Arducam 3MP, 5MP, OmniVision 5640) for low-cost, high-sensitivity CLIA applications.
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